Plant root exudates are considered to be an important communication system between plants and the soil microenvironment. CD BioSciences is committed to developing a variety of methods for the collection, purification and detection of root exudates to provide clients with analysis services of root exudates. We will formulate the optimal research strategy of root exudates according to the specific scientific research needs of clients.

Introduction

During plant growth and development, the root system is not only an absorptive and metabolic organ, but also a powerful secretory organ. These compounds, including sugars, organic acids, amino acids, phenolics, polypeptides and proteins, alcohols, hormones and enzymes, which are secreted by plant roots into rhizosphere soil, are called root exudates. They are an important medium for the exchange of material, energy and information between the plant and the soil microenvironment. Therefore, the analysis of root exudates is critical for research in the fields of plant nutrition, chemosensory effects and environmental remediation.

The uptake and utilization of root secretions by microorganisms in the soil, as well as the complexity and low content of root secretion components, make it difficult to extract and analyze root exudates effectively. Modern approaches to the study of plant-microbial interactions generally rely on analytical techniques to decipher the complexity of root exudate components. Rapid advances in the field of metabolomic analysis offer the possibility of isolating, characterizing and quantifying root-exuded metabolites.

Fig.1 Scheme of MALDI MSI of roots. (Casas, 2021)

Our Services

We provide clients with a one-stop service for analyzing root exudates, including the collection, isolation and purification, and determination of root exudates. Our state-of-the-art technology platform features a wide range of qualitative and quantitative analyses by mass spectrometry, providing a powerful tool for a comprehensive understanding of root exudates. Our services include but are not limited to:

• Collection of root exudates
• Isolation and purification of root exudates
• Detection of root exudates
  ➢ Structural analysis tests
  We use a variety of biophysical technologies to help clients determine the functional groups, conjugated systems, the combination mode of hydrogen atoms and carbon atoms in molecules, and obtain the molecular weight and structure of the substance to be tested, and then determine chemical structures.
  ➢ Component analysis tests
  We identify the exudate components such as phenols, aromatic hydrocarbons, esters, amines, etc. with high resolution and sensitivity using a variety of techniques such as mass spectrometry and chromatography combined.

Our Advanced Biophysical Analysis Platform

• Mass spectrometry (MS) techniques
• Gas chromatography (GC) coupled with MS
• Liquid chromatography (LC) coupled with MS
• Capillary electrophoresis (CE) with MS
• Ion mobility MS
• Direct-injection MS
• MALDI MS and MS imaging

With a dedicated and highly qualified research team, CD BioSciences can develop optimal strategies for the study of root exudates for our clients. We provide services for the detection and analysis of root exudate quantity, species and structure through efficient biophysical analysis methods. If you are interested in our services, please feel free to contact us for more details.

Reference

1. Casas, M. E.; Matamoros, V. Analytical challenges and solutions for performing metabolomic analysis of root exudates. Trends in Environmental Analytical Chemistry. 2021, 31: e00130.

• Detection of Root Phenotype
• Measurement of Root Physiological Characteristics
• Geometric Characterization of Developing Lateral Roots
• Analysis of Root Growth Under Mechanical Stress
• Modeling of Root Growth
• Modeling of Root-Soil Interactions
• AFM-Based Biophysical Analysis of Root Growth