Antibody Structure Characterization
With the development of biopharmaceuticals, comprehensive characterization of the key quality attributes of antibodies has become the focus and difficulty in antibody drug development. As a biophysical expert, CD BioSciences is committed to providing a variety of advanced biophysical analysis techniques for the comprehensive characterization of antibody structure. Our services cover from preliminary characterization to detailed high-resolution structural analysis, further helping customers make important progress in antibody research and development.
Background
In recent years, monoclonal antibodies (mAbs) have rapidly become a successful class of biologics for tumor targeting and immunotherapy. The efficacy of mAbs is closely related to their structure. Accurate antibody structural information can not only reveal its mechanism of action but also guide the design, optimization, and clinical application of antibody drugs. Ensuring the structure of complementary determining regions (CDRs) so that they can bind to antigens is the key to the efficacy of mAbs. With the rapid development of biophysical technology, high-resolution structural analysis methods continue to emerge, allowing us to explore the three-dimensional structure and dynamic behavior of antibodies.
Figure 1. Therapeutic monoclonal antibodies crystallize. (Brader M L, et al., 2017)
Our Services
X-ray crystallography is the gold standard method for analyzing high-resolution three-dimensional structures of antibodies. Through this technology, we can provide atomic-level structural information to help customers understand the mechanism of action of antibodies and their interaction with antigens. Our services include high-resolution structural elucidation, complex sample processing, automated crystal screening, and data processing and modeling to ensure the accuracy and reliability of the results.
- Nuclear Magnetic Resonance (NMR) Spectroscopy
NMR spectroscopy is a powerful tool for studying the native conformation of antibodies in solution and their dynamic behavior. We provide services for kinetic and conformational studies, and a wide range of samples, and can handle antibodies and complexes up to 150 kDa. In addition, through multidimensional NMR technology and isotope labeling, we can obtain higher resolution and more detailed structural information.
- Surface Plasmon Resonance (SPR)
SPR technology is used to monitor the interaction between antibodies and antigens in real time, providing binding kinetics and affinity data. Our service features include real-time interaction analysis without labeling, high-sensitivity detection, multi-channel analysis, and detailed kinetic parameters (such as association rate and dissociation rate), providing important reference data for drug discovery and development.
- Differential Scanning Calorimetry (DSC)
DSC technology is used to measure the thermal stability of antibodies, helping to evaluate the thermodynamic properties and denaturation temperature (Tm) of antibodies. Our services include stability analysis, widely applicable sample processing, high-throughput screening, and quantitative analysis, providing customers with detailed thermodynamic data on antibody stability.
Our platform uses Size Exclusion Chromatography coupled with Multi-Angle Light Scattering (SEC-MALS), hydrophobic interaction chromatography (HIC), ion exchange chromatography (IEX), and other technologies for antibody structural characterization.
Application Areas
- Antibody drug development. Our team provides accurate structural information to guide antibody design and optimization and improve the efficacy and safety of drugs.
- Antibody structural function research. We reveal the mechanism of action and antigen recognition mode of antibodies through a variety of biophysical techniques.
- Antibody stability evaluation. By evaluating the stability of antibodies under different conditions, we can further provide a reference for formulation development.
Our Advantages
- Strong technical support. Our biophysicists have rich experience in antibody structure characterization and can provide professional guidance to our customers.
- Advanced analytical equipment. Our platform is equipped with a range of professional equipment for antibody characterization, ensuring that we can provide prepared data to our clients.
- Short cycle time. We tailor our experimental programs to meet our client's needs and deliver results in a short period.
CD BioSciences is committed to providing the highest quality service and support for your research. If you are interested in our services, please feel free to contact us.
Reference
- Brader M L, Baker E N, Dunn M F, et al. (2017). Using X-ray crystallography to simplify and accelerate biologics drug development[J]. Journal of Pharmaceutical Sciences. 106(2): 477-494.
For research use only, not
intended for any clinical use.
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