In the field of biopharmaceuticals, antibodies, as important therapeutic proteins, have thermal stability that is critical to their potency and quality. As the development and production of biologics become increasingly complex, it is particularly important to ensure the structural and functional stability of antibodies under different temperature conditions. CD BioSciences offers advanced biophysical technologies to provide customers with efficient and accurate antibody thermal stability assessment services, helping them to accelerate drug discovery and optimize antibody design and production.

Background

Antibody activity is the most critical quality attribute for its therapeutic effect, among which thermal stability evaluation is an important part to reflect the correctness of the advanced structure, and high thermal stability is also the guarantee for the drugability and storage of antibody drugs. Currently, a variety of biophysical techniques such as differential scanning calorimetry (DSC) and dynamic light scattering (DLS) are capable of comprehensively evaluating the thermal stability of antibodies. These techniques can accurately determine the stability and denaturation temperature of antibodies under different temperature conditions, thus providing a scientific basis for antibody development and optimization.

Figure 1. Thermal unfolding of mAb in 1.0 M guanidine HCl at pH 6.2, measured with differential scanning calorimetry (DSC). (Garidel P, et al., 2020)

Our Technologies

• Differential Scanning Calorimetry (DSC)

We offer DSC technology for high-precision thermodynamic measurements of antibodies, which accurately determines the melting temperature (Tm) of an antibody and identifies the thermal stability and denaturation temperature of an antibody. With comprehensive thermodynamic profiles, we can analyze the stability changes of antibodies during warming.

• Differential Scanning Fluorimetry (nanoDSF)

The nanoDSF technology detects thermal and chemical denaturation of proteins under natural conditions. Label-free nanoDSF technology can accurately detect changes in endogenous fluorescence during thermal and chemical denaturation of proteins. We have developed a nanoDSF technology platform that enables the determination of the thermal or chemical stability of antibodies in a non-labeled environment by detecting changes in fluorescence and ensuring that data quality is not affected by sample aggregation.

• Dynamic Light Scattering (DLS)

To analyze particle size and aggregation states, we use DLS to detect particle size changes in antibodies at different temperatures and to identify aggregation and depolymerization behavior. This process eliminates the need for complex sample processing, preserves the original state of the antibody, and is suitable for a wide range of sample types.

• Thermofluorescence Analysis (TFA)

Our team uses TFA technology to rapidly determine the fluorescence intensity of an antibody under temperature changes, providing quantitative data on the relative thermal stability of the antibody through changes in fluorescence signal.

• Isothermal Titration Calorimetry (ITC)

Our ITC technology not only determines the binding constants of antibodies but also provides thermodynamic parameters of the binding process, further helping to understand the thermal stability of antibody-antigen interactions.

Our Service Process

• Sample processing. Customers provide samples of antibodies to be tested, and we perform an initial quality check to ensure that the samples are suitable for further testing.
• Technology selection and program development. We recommend the most suitable biophysical technology and develop a detailed test protocol based on the client's needs and sample characteristics.
• Experimental stage. We conduct accurate data collection through advanced instrumentation, and subsequently record the performance of the antibody under different temperature conditions.
• Data analysis. Our team conducts an in-depth analysis of the experimental data and generates a comprehensive thermal stability assessment report containing key indicators and conclusions.
• Results feedback. We provide detailed technical reports and follow-up technical consultation and support services to our clients.

Our Advantages

• Advanced technology platform. Our laboratory is equipped with first-class biophysical analyzers to ensure the accuracy and reliability of data.
• Strong technical support. Our team consists of experienced experts who are committed to providing professional technical support and consulting services to our clients.
• Customization. We will provide customized evaluation solutions to meet the needs of different R&D stages.

CD BioSciences, as a leading biophysical company, is committed to providing customers with a variety of biophysical techniques to comprehensively evaluate the thermal stability of antibodies. Through professional experimental design and accurate data analysis, we will help our clients gain a deeper understanding of the structural stability of antibodies under temperature changes and the factors that influence them. If you are interested in our services, please contact us for more details.

Reference

1. Garidel P, Eiperle A, Blech M, et al. (2020). Thermal and chemical unfolding of a monoclonal IgG1 antibody: application of the multistate Zimm-Bragg theory[J]. Biophysical journal. 118(5): 1067-1075.

• Antibody-Antigen Interaction Analysis by BLI
• Antibody Structure Characterization
• Antibody Particle Size Distribution and Aggregation Analysis