Equipped with a first-class biophysical technology platform, CD BioSciences is focusing on providing clients with professional cardiac mitochondrial characterization services by atomic force microscopy (AFM) and other technologies. We help our clients analyze and quantify the morphological and property changes of heart mitochondria in a variety of animal models to accelerate their heart-related biomedical research.

Background

Mitochondria are a major source of energy in the heart and play an important role in cell death and cardioprotection. The role of mitochondria in myocardial ischemia-induced injury is complex. It has been shown that mitochondrial dysfunction leads to the following events: loss of ATP synthesis and increased ATP hydrolysis, impaired homeostasis, formation of reactive oxygen species (ROS) and release of pro-apoptotic proteins, ultimately leading to myocardial infarction by causing irreversible damage to cell viability.

Changes in mitochondrial morphology, such as swelling and clumping, are closely linked to a wide range of important biologic functions and pathologies. Therefore, the characterization of mitochondrial biophysical properties such as quantitative analysis of morphological changes can help to assess the extent of myocardial damage. As mitochondrial swelling is an ultrastructural change, high-resolution microscopic methods such as AFM or electron microscopy (EM) are required, which can accelerate the study of the pathological processes involved in myocardial injury.

Fig.1 Representative AFM phase images of mitochondria isolated from normal (a) and ischemic (b) hearts. (Lee, 2011)

Our Services

We offer a wide range of biophysical analysis techniques such as AFM to help our clients characterize the biophysical characteristics of heart mitochondria and our analytical services are available for a wide range of animal models. Our services include but are not limited to:

• Shape analysis of heart mitochondria to characterize the nanostructural changes resulting from myocardial IR injury.
• Quantitative evaluation of mitochondrial morphological differences between normal and ischemic hearts by measuring the shape parameters, including their areas, perimeters, lengths, breadths, and aspect ratios.
• Force-distance analysis to reveal the viscoelastic changes of mitochondria in ischemic myocardium.
• Cryo-EM structural analysis of mitochondrial complex I (NADH: ubiquinone oxidoreductase).
• Analysis of iron metabolism in heart mitochondria.

Applications

• Study on the pathology of myocardial infarction.
• Evaluation of the degree of myocardial injury.
• Development of mechanistic models for mammalian complex I.

Our Advantages

• Latest tools and techniques.
• High-quality data and customer services.
• Expertise with competitive pricing and rapid turnaround.

CD BioSciences is a leading biophysical analysis service provider in the world, including providing high-quality cardiac mitochondrial characterization services for academia and the biotechnology industry. The innovative, comprehensive and time-saving scientific services we provide will make an essential contribution to your research on the pathological mechanism of diseases such as myocardial injury. If you need our scientific support, please contact us for more details.

Reference

1. Lee, G. J.; et al. Characterization of mitochondria isolated from normal and ischemic hearts in rats utilizing atomic force microscopy. Micron. 2011, 42(3): 299-304.

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• High-Resolution Imaging and Characterization of Various Biomolecules