As a provider of high-quality biophysical analysis services, CD BioSciences offers a wide range of advanced techniques to help clients study the interactions between cellulose and matrix polysaccharides, providing useful information for further understanding of cell wall structure and assembly. Our dedicated research team will provide optimal solutions and scientific services for each customer.

Background

Exploring the interactions of cellulose with other matrix polysaccharides is key to linking cell wall structure to cell growth and mechanics. However, due to the lack of high-resolution techniques for characterizing the molecular structure, dynamics and intermolecular interactions of polysaccharides in the cell wall, little is known about how cell wall polymers form 3D networks to provide mechanical strength to the cell wall while allowing cell wall expansion and growth.

In the last few decades, structural characterization of cell walls has mainly involved chemical extraction followed by glycoanalysis and microscopic imaging, an approach limited by significant perturbations in wall structure and insufficient spatial resolution. In addition, in vitro binding assays have been used to measure the binding affinity between different wall polysaccharides, but this method does not reproduce the complex molecular interactions in natural walls after biosynthesis. Current advances in biophysical techniques have led to the development of a variety of new methods including scattering, spectroscopy and microscopy techniques such as AFM and FESEM that can be used to examine the interaction of cellulose and matrix polysaccharides.

Fig.1 2D ¹³C-¹³C proton-driven spin diffusion (PDSD) spectrum of Arabidopsis cell walls with a 1.5 s mixing time. (Wang, 2016)

Our Services

To help our customers obtain critical information about the interaction between cellulose and matrix polysaccharides, we have developed a variety of biophysically based analytical techniques including field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), small-angle X-ray scattering (SAXS), IR, Raman, sum-frequency generation (SFG), and 2D and 3D correlated solid-state NMR (SSNMR) spectroscopy. Our services include, but are not limited to:

• Analysis of the spatial arrangement and conformation of cell wall polysaccharides in the natural cell wall.
• Estimation of the number of glucan chains in cellulose microfibers.
• Analysis of how matrix polymers such as xylan, xyloglucan, mannans and pectins affect cellulose crystallization and how cellulose interacts with matrix polysaccharides.
• Analysis of site-specific information on the conformation, dynamics, water interactions and intermolecular contacts of macromolecules in the cell walls of near-native plant cell walls.

Our Advantages

• Advanced analytical techniques and data processing methods
• Fast and traceable detection services
• Most competitive price and customized service

CD BioSciences offers new biophysical analysis techniques for the detection of interactions between cellulose and matrix polysaccharides, enabling research teams to gain insight into the structure and characterization of cellulose. We use cutting-edge technology to provide highly skilled and professional services to our clients. If you are interested in our services, please contact us for further inquiries.

Reference

1. Wang, T.; Hong, M. Solid-state NMR investigations of cellulose structure and interactions with matrix polysaccharides in plant primary cell walls. Journal of experimental botany. 2016, 67(2): 503-514.

• Detection of Unit Cell Parameters of Cellulose
• Measurement of Cellulose Crystallinity Index
• Morphological Characterization of Cellulose Microfibres