CD BioSciences offers multiple methods to provide our clients with electrophysiological measurements of neurons. Our professional technical services will make an important contribution to your exploration of the electrical activity of living neurons and the study of the molecular and cellular processes that control their signaling. We look forward to becoming your reliable partner.

Introduction

Electrophysiology includes the measurement and manipulation of voltages and currents from single ion channels to whole organs. In the field of neuroscience, the electrical recording and analysis of nerves are fundamental means of studying the processes of neural activity, as neurons communicate using electrical and chemical signals. Electrophysiological techniques listen to these signals by measuring electrical activity, enabling scientists to decode intercellular and intracellular information.

The creation of microelectrode technology has greatly advanced the understanding of neuroelectricity. With the development of computer technology and highly sophisticated electronic devices, the study of neurophysiology has entered a period of rapid development. Currently, neuroelectrophysiological recording techniques have become an important tool for studying neural function, and these techniques can answer system-level questions such as the role of neurons in neural circuits or behavior. Alternatively, they can be used to study the specific ion channels, membrane potentials and molecules that give each neuron its physiological characteristics.

Fig.1 Some of the fundamental components of an electrophysiology. (Carter, 2015)

Our Services

At CD BioSciences, the main recording methods for electrical nerve signals are extracellular recordings, intracellular recordings and patch-clamp recordings. With these three techniques, we offer our clients the following services:

• Measurements of electrical potentials and currents from the entire cell.
• Recording of the frequency of action potential changes in a particular neuron over time.
• Quantification of the number of action potentials, or spikes, that a neuron fires in response to a specific stimulus.
• Analysis of the effect of the activity (or inactivity) of one neuron on the local and action potentials of another neuron.
• Analysis of the effects of pharmacological agents, neurotransmitters and neuromodulators on the local and action potentials of neurons.
• Record the current carried by a single ion channel.
• Analysis of the opening and closing times of ion channels in relation to membrane potential.
• Analysis of the effect of the concentration of ions, pharmacological agents, neurotransmitters and neuromodulators on the current flowing into an ion channel or cell.

Applications

• Research on the mechanisms by which neurons encode specific stimuli or actions.
• Research on the mechanisms of coordination of spiking activity in a group of neurons.
• Research on the ionic basis of neurons.

With an advanced technology platform, CD BioSciences has established a variety of techniques including extracellular recordings, intracellular recordings and patch-clamp recordings to provide our clients with electrophysiological measurements of neurons. Our dedicated research team will develop the optimal solution for your project. If you are interested in our services, please feel free to contact us.

Reference

1. Carter, M.; Shieh, J. C. Guide to research techniques in neuroscience. Academic Press. 2015.

• Whole-Brain Imaging Based on Biophysical Technology
• Detection of Neurotransmitters
• Analysis of Neurotransmitter Release
• Analysis of Synaptic Transmission and Plasticity
• Analysis of 3D Neural Morphology
• Visualization of Neural Activity
• Structural and Functional Analysis of Neural Circuits
• Biophysical Modeling of Neural Systems