The dissociation/deconstruction of the different components is a prerequisite for the analysis of ex-situ cell wall components. CD BioSciences offers our customers a variety of chemical, enzymatic, and physical methods to accomplish linkage breakdown between polymers. We aim to release all cell wall components without altering their chemical composition.
Plant cell walls are complex 3D networks composed of proteins, polysaccharides, and lignin. Multiple molecular interactions make the analysis of cell wall compounds a more complex task. In order to analyze ex-situ cell wall components, their destructuration /dissociation/disorganization from each other is a prerequisite for their analysis. The dissociation of plant cell walls depends heavily on the number and type of bonds (covalent, electrostatic, hydrogen, ionic linkage) between them. The disintegration of bonds between these polymers can be achieved by chemical, physical, enzymatic, or by a mixture of two or three of these methods. Developing efficient and rapid methods to release all cell wall components without altering their chemical compositions is currently a major challenge in the in vitro analysis of cell wall components.
Fig.1 Diagrammatic representation of the major structural polysaccharide components of a "typical" primary plant cell wall. (Flint, 2012)
Researchers at CD BioSciences have developed a variety of methods for purifying plant cell wall polymers to help clients characterize cell wall composition and to lay the experimental foundation for understanding the function of different cell wall polymers in plant development and adaptation. Our services include but are not limited to:
With a specialized biophysical analysis platform, CD BioSciences offers a full range of cell wall components characterization services, including pre-analytical cell wall component extraction. If you need our technical support, please contact us and we will be happy to develop the best experimental protocol for you.
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CD BioSciences is a biotechnology company focused on biophysical services. We are proficient in both chemistry and biophysics, and have a comprehensive biophysical platform containing a wide range of advanced technologies.