Glycan Profiling of Plant Cell Wall Polymers
With an in-depth understanding of plant cell wall structure, CD BioSciences is committed to continuously developing and optimizing new methods to improve structural characterization and analytical capabilities. Our flexible solutions will help our customers to perform glycan profiling of plant cell wall polymers to facilitate in-depth understanding of plant cell walls.
Background
The plant cell wall is a complex matrix containing heterogeneous glycans, which controls the morphology, growth and development of plants. It is the plant's first barrier against a variety of biotic/abiotic stresses. Despite the important role that the cell wall plays in plant development, understanding the structural characteristics and functions of its components remains challenging due to the limitations of available technologies.
The complexity of the structural units of cell wall glycans, the glycosyl residues, results in their conformational and chemical diversity. Some studies have shown a link between alterations in wall glycans and disease resistance in crops. Given the important role of wall glycans in plant immunity, it is necessary to establish new methods to characterize the different ranges of glycan structures. Monoclonal antibodies (mAb) developed against cell wall polymers are now an important tool for determining the structure and distribution of glycans in plant cell walls.
Fig.1 Glycan profiling of Nicotiana alata flower tissues represented pictorially. (Moller, 2012)
Our Services
In order to determine the structure and distribution of glycans in plants, we have developed a microarray-based glycan screening method called Comprehensive Microarray Polymer Analysis (CoMPP) to track glycan changes in complex biological systems. We apply carbohydrate microarray technology in combination with other classical methods for cell wall analysis (GC-MS and FTIR) to provide our clients with a service for glycan profiling of plant cell wall polymers. Our services include but are not limited to:
- We use CoMPP to obtain semi-quantitative data on the occurrence of glycan epitopes.
- We have extended the use of CoMPP to large-scale screening experiments to determine the relative abundance of glycans in multiple plants and tissue types simultaneously.
- We offer GC-MS to provide additional monosaccharide composition data for validation of CoMPP analyses.
- We offer FTIR spectroscopy for quick and easy detection of changes in chemical functional groups in cell wall samples to support the results of carbohydrate microarray experiments.
Advantages
- The serious and rigorous working attitude
- Detection with high sensitivity and high-throughput
- Powerful analysis software
- Detailed and comprehensive analysis reports
The highly qualified experts team of CD BioSciences has the potential to develop more strategies for glycan profiling of plant cell wall polymers to help researchers better understand the biological properties of cell walls. Our high-quality scientific services will meet your research needs and accelerate your research progress. If you are interested in our services, please contact us and we look forward to being your reliable partner.
Reference
- Moller, I. E.; et al. Glycan profiling of plant cell wall polymers using microarrays. Journal of Visualized Experiments. 2012 (70): e4238.
For research use only, not
intended for any clinical use.
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