Advances in imaging technology have opened up the possibility of visualizing individual actin filaments in real-time. CD BioSciences is committed to improving and optimizing experimental strategies to provide our clients with high-resolution imaging of the actin cytoskeleton in pollen tubes. Our professional scientific services and technical support will contribute to your research in the field of pollen tip growth.

Background

An intact actin cytoskeleton is necessary for plants to maintain normal physiological processes such as cell morphogenesis, cell wall construction and signal transduction. Pollen tube elongation is a polar cell growth process that is heavily dependent on the regulation of the active actin cytoskeleton. Although actin has been shown to have an important role in the regulation of pollen germination and pollen tube growth, inadequate knowledge of the spatial organization and dynamics of actin filaments within the pollen tube growth domain has limited our understanding of how actin exerts its function.

For many years, actin has been the focus of numerous researchers who have attempted to establish a consistent and clear pattern of actin organization, particularly in the apex of the pollen tube. However, the results have varied greatly due to the problems of fixing very fast-growing cells and the fact that actin microfilaments are very fragile and therefore easily disrupted. Recent advances in live-cell imaging in the physical realm have opened up the possibility for researchers to understand the precise molecular mechanisms underlying actin cytoskeleton function in pollen tubes.

Fig.1 Schematic diagram of the regulation of actin polymerization and dynamics in the pollen tube. (Xu, 2020)

Our Services

CD BioSciences focuses on building complex Spatio-temporal imaging techniques and developing suitable actin markers to visualize individual actin filaments. Advanced imaging techniques allow us to provide our customers with efficient technical support in studying the dynamics of actin filaments in pollen tubes. The services we provide include but are not limited to:

• Imaging the actin cytoskeleton in pollen tubes
  We provide our clients with a complete view of the actin cytoskeleton in pollen tubes by rapid freeze fixation followed by immunostaining. This method provides key information on the spatial distribution of actin filaments in pollen tubes.
• Imaging of the actin cytoskeleton in growing pollen tubes
  We help our clients develop effective live-cell probes to detect the presence of actin arrays in growing pollen tubes with minimal interference with normal cellular actin dynamics. This strategy allows the dynamics of actin filaments to be tracked during pollen tube growth and helps to reveal the remodeling of the pollen tube actin cytoskeleton during pollen tube growth.

Our Advantages

• A comprehensive and advanced platform
• Optimization of the strategy according to the client's project requirements
• Powerful data analysis software
• Short experiment period

CD BioSciences is a technology company focused on providing biophysical analysis services. We combine advanced imaging techniques to help our clients obtain detailed information on the dynamic properties of actin filaments in the apical region. If you are interested in our services, please contact us and we will provide you with comprehensive, high quality and affordable services.

Reference

1. Xu, Y.; Huang, S. Control of the actin cytoskeleton within apical and subapical regions of pollen tubes. Frontiers in Cell and Developmental Biology. 2020: 1501.

• Analysis of Pollen Tube Growth Behavior
• Determination of Pollen Tube Extension
• Analysis of Pollen Hydration
• Live Cell Imaging of Pollen Tubes
• Measurement of Cytomechanical Forces on Growing Pollen Tubes
• Modeling of Pollen Tube Growth
• AFM-Based Biophysical Analysis of Pollen Tubes