As a biotechnology company specializing in biophysical analysis, CD BioSciences offers a variety of nuclear magnetic resonance (NMR) techniques to help clients explore the structure of photosynthesis and analyze lipid polymorphisms in chloroplast thylakoid membranes and lipid conformational dynamics. Our professional research team will provide you with first-class scientific services.

Introduction

Intact membrane proteins are exposed to a complex and dynamic lipid environment regulated by nonbilayer lipids, which can have an impact on protein functions through lipid-protein interactions. In plant photosynthetic thylakoid membranes, the most abundant lipid is the non-bilayer lipid monogalactosyl diacylglycerol (MGDG), but no studies have yet clearly elucidated the effect of MGDG on the function of energy-converting membrane protein complexes.

Exploring the physical properties of thylakoid membrane lipids has implications for photosynthesis research in several areas, such as lipoprotein interactions in the context of the structure and function of major photosynthetic membrane complexes, lipid phase behavior and its relevance to membrane phase separation, and membrane fluidity in terms of thermal adaptation and factors limiting electron transport rates. Therefore, a deeper understanding of lipid polymorphism in chloroplast thylakoid membranes and the conformational dynamics of lipids is of great importance for the study of photosynthesis.

Fig.1 ³¹P-NMR spectra of isolated spinach thylakoid membranes. (Garab, 2017)

Our Services

Equipped with a specialized biophysical analysis platform, we can provide our clients with a variety of techniques to accelerate their research, such as ³¹P-NMR, time-resolved merocyanine fluorescence spectroscopy, in situ ¹³C solid-state NMR, and magic angle spinning (MAS) NMR techniques. The services we offer include but are not limited to:

• Analysis of lipid polymorphism in chloroplast thylakoid membranes.
• Detection of protein, lipid and xanthophyll molecular constituents in photosynthetic thylakoid membranes.
• Molecular dynamics of proteins and lipids in photosynthetic thylakoid membranes.
• Isolation and quantification of rigid and dynamic membrane molecular compositions.

Applications

• Study of the polymorphic phase behavior of lipids
• Structural dynamics studies of thylakoid membranes
• Design of future artificial photosynthetic structures

Advantages of Our Services

• Experienced and creative professional R&D team
• High-quality services and fast delivery cycle of data results
• Rigorous experimental control system and accurate characterization data

CD BioSciences has rich experience and a professional research team, as well as a perfect service process and quality assurance system. Relying on advanced biophysical techniques, we provide reliable services for the conformational dynamics analysis of lipids in chloroplast thylakoid membranes. If you need our services, please contact us and we will develop an experimental strategy/methodology according to your requirements.

Reference

1. Garab, G.; et al. Lipid polymorphism in chloroplast thylakoid membranes – as revealed by ³¹P-NMR and time-resolved merocyanine fluorescence spectroscopy. Scientific reports. 2017, 7(1): 1-11.

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• Plants Magnetic Resonance Imaging Services
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• Structural Characterization of Amorphous Photosynthetic Complexes
• Kinetic Analysis of Pigment-Protein Complex
• Distance Measurements in Photosynthetic Reaction Centers
• Molecular Dynamics Simulations in Photosynthesis