Protein engineering is a technology that combines protein crystallography and computer-aided design techniques to modify, remodel, and splice proteins to produce novel proteins that can meet the needs using artificial targeted modification of genes and other means. Understanding protein structure and function is crucial for drug discovery and disease treatment, providing new directions and ideas for drug development. Our biophysical techniques can predict the structure of proteins and express the desired functional protein molecules through modification, transformation, and molecular design, which is important in drug discovery and agricultural production.
CD BioSciences is dedicated to the research and analysis of proteins, with a comprehensive biophysical technology platform to reveal the structure and function of proteins to promote the development of biomedicine.
Figure 1. Selection of different biophysical methods aimed to determine structural and functional information of multidrug transporter EmrE bound to lipids. (Montenegro F A, et al., 2017)
Applications in Protein Engineering
CD BioSciences has successfully provided biophysical analysis services to several research organizations, helping to achieve remarkable results in the field of protein engineering. We have significantly improved the efficacy of antibodies by optimizing their stability. In addition, the structural analysis of a variety of enzymes using our technology has given important clues to the study of their function in metabolic pathways.
Protein Folding
When protein folding malfunctions in the body and forms incorrect spatial structures, it not only loses its biological function, but also causes diseases, such as Alzheimer's disease, cystic fibrosis, and familial amyloidosis. We provide specialized biophysical techniques such as X-ray diffraction, spectroscopy, mass spectrometry, and nuclear magnetic resonance (NMR) to study the protein folding process. Through the simulation of the protein folding process, we can further investigate the movement of biological macromolecules, the investigation of protein function, and the rationalization of industrial enzyme design.
Protein Structure
To fully understand the functions and mechanisms of proteins, we have deployed X-ray crystallography, electron microscopy, and nuclear magnetic resonance techniques to reveal the structures of proteins and to advance disease mechanisms and drug discovery further. In addition, we combine molecular and computational simulation techniques to predict the 3D structure of proteins. By analyzing the structure of proteins and identifying functional units or structural domains, we can provide targets for gene manipulation, a reliable basis for designing new proteins or modifying existing proteins, and rational target molecules and structures for new drug molecule design.
Protein Stability
Stability is the tendency of a protein to maintain its natural conformation. Disulfide bonds in proteins are important structural elements, and their formation and stabilization mechanisms are crucial for maintaining the correct folding and function of proteins. Disulfide bonds enhance the structural rigidity of proteins, thereby improving their resistance to change and thermal stability. Our technology platform has biophysical techniques such as mass spectrometry, nuclear magnetic resonance, and X-ray crystallography to obtain high-resolution information on protein structure so that we can directly observe the formation of disulfide bonds, and thus help our customers to design more protein-stabilizing drugs and improve their efficacy and safety.
Protein Interactions
We can provide isothermal titration calorimetry (ITC) to study the interactions of protein molecules by measuring the heat exchange process between them. Based on this, we can offer thermodynamic parameters of protein interactions such as thermodynamic equilibrium constants, enthalpy, and entropy changes. The study of protein-ligand interactions, protein-protein interactions, and enzymatic reactions helps us understand biomolecules' functions and regulatory mechanisms, which can guide drug design and therapeutic strategies.
Advantages of Our Services
- All-rounded technology platform. We are equipped with advanced instrumentation and technical means for protein engineering research, dedicated to providing clients with accurate data reports.
- Powerful specialty. We possess an experienced and skilled professional team with a profound background in biophysical analysis and rich practical knowledge.
- Perfect program. We will integrate clients' needs to develop a professional and customized service process.
Related Services
CD BioSciences has extensive experience in the biophysical field, with our complete technology platform and specialized research team providing diverse services and solutions for protein engineering. If you are interested in our services, please contact us.
Reference
- Montenegro F A, Cantero J R, Barrera N P. (2017). Combining mass spectrometry and X-ray crystallography for analyzing native-like membrane protein lipid complexes[J]. Frontiers in Physiology. 8: 300674.
For research use only, not
intended for any clinical use.
Related Services
