Surface Plasmon Resonance (SPR)
Introduction
Surface plasmon resonance (SPR) is a biophysical technique developed for the detection of molecular interactions based on the principles of physical optics, which allows for the detection of intermolecular interactions in real-time with high sensitivity and without the need for labeling. SPR has been recognized as one of the most powerful techniques for determining the specificity, affinity, and kinetic parameters of a wide range of molecular binding processes of many types, including protein-protein, protein-DNA, enzyme-substrate or inhibitor, etc. The technology can be used for protein interaction studies, small and large molecule drug discovery, antibody screening, and precise characterization, which has been widely used in life sciences, medical diagnostics, food safety, and environmental testing.
Figure 1. Experimental setup of surface plasmon resonance (SPR) biosensor, (Firdous S, et al., 2018)
Working Principle
SPR technology generates different resonance angles according to whether the ligand coupled to the metal surface binds to the analyte or not. By monitoring the change of the resonance angle, the affinity, affinity constants, binding kinetics, and other information of biomolecular interactions can be inferred.
Features
- Real-time detection can dynamically monitor the whole process of biomolecular interactions.
- No need to label the sample, maintaining the molecular activity.
- Convenient and fast detection process with high sensitivity.
- High throughput and high-quality analysis data.
- Ability to track and monitor the stability of immobilized ligands.
CD BioSciences owns comprehensive biophysical analysis platforms committed to providing surface plasmon resonance (SPR) services for drug discovery, biosensor development, and biomolecular interaction studies.
Main Applications
In the early stage of antibody drug development, SPR technology can be utilized for target antibody screening to identify candidate molecules. In the production stage, we provide professional SPR services to complete the characterization of antibody drugs, including epitope analysis, affinity analysis, and active substance quantification. In addition, we can accelerate the drug development process by evaluating the binding affinity and kinetic properties of the drug to the target in terms of quality control.
- Biomolecular Interaction Research
SPR technology can be used to study the interactions between biomolecules such as proteins, nucleic acids, and drugs, including ligand-receptor, protein-protein, and antibody-antigen. We can obtain high spatial and temporal resolution information by monitoring the binding and dissociation processes of biomolecules in real time using SPR technology. Due to the high sensitivity to binding events of biomolecules, we can detect trace molecular interactions.
Biosensors constructed based on the SPR principle can be used to detect biomolecules, cells, viruses, etc., with the advantages of real-time, high sensitivity, and high specificity. This advantage makes it widely used in the fields of medical diagnosis and environmental monitoring.
What Can We Offer?
CD BioSciences endeavors to offer surface plasmon resonance (SPR) services to accelerate our customer's research process. If you are interested in our services, please feel free to contact us.
Reference
- Firdous S, Anwar S, Rafya R. (2018). Development of surface plasmon resonance (SPR) biosensors for use in the diagnostics of malignant and infectious diseases[J]. Laser physics letters. 15(6): 065602.
For research use only, not
intended for any clinical use.
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