Hydrogen/deuterium exchange mass spectrometry (HDX-MS) is an important tool in the research of small molecule drugs and biopharmaceuticals. CD BioSciences is committed to developing and applying HDX-MS in the field of drug discovery to help our clients obtain a detailed evaluation of the participation of biological candidate drug targets. Our target binding analysis service will provide support for the development decisions of complex biologics.

Background

HDX-MS is a novel mass spectrometry technique to study the advanced structure of proteins and has a wide range of applications in the study of proteome structure and dynamic changes in protein epitopes and active site identification. Scientists often use combinatorial analysis to accomplish a high-resolution analysis of details of interactions to reduce risks and facilitate early drug development by assessing target engagement and kinetics.

Combined with the latest MS techniques and data analysis software, HDX-MS technology can be used to map the interaction of binding sites. This technology has the advantages of low requirements for protein purity, no crystallization and only a small number of samples. In addition, this method can provide a physiological conformation that better reflects the true state of the protein. HDX-MS is used in a wide range of biopharmaceutical applications, from drug discovery to functional validation, including the study of protein conformation, modification-induced dynamics, folding, protein degradation and protein/protein interactions.

Fig.1 Diagram of an accelerated HDX-MS epitope screening workflow. (Zhu, 2022)

Our Services

We provide our clients with target binding analysis by HDX-MS and tailor analytical procedures to best suit their specific scientific needs. Our services include but are not limited to:

• Identification of binding regions and assessment of binding mode.
• Epitope mapping for binding site identification.
• Assessment of higher-order structure (HOS).
• Determination of target binding stoichiometry.
• Paratope mapping for identification of the binding site and conformational changes.
• Protein folding characterization.
• Biosimilar characterization.
• Analysis of conformational/protein dynamics.

Advantages of Our Technology Platform

• Automated pre-treatment and injection analysis with precise control of hydrogen-deuterium-atom exchange reaction times.
• State-of-the-art equipment: using the latest mass spectrometry, automated robotics and analysis software.
• Extensive, timely and interactive results management.
• Applicable for conformational, discontinuous, linear epitopes and many other conformational protein studies.

How to order?

Based on over a decade of expertise in MS characterization of protein interactions and protein complexes, CD BioSciences provides high-quality target binding analysis services to clients. With HDX-MS, we provide our clients with reliable results in a predetermined timeframe, so as to provide unique expertise in the field of drug discovery. If you are interested in our services, please contact us directly and we will respond to you as soon as possible.

Reference

1. Zhu, S.; et al. Epitope screening using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX‐MS): An accelerated workflow for evaluation of lead monoclonal antibodies. Biotechnology journal. 2022, 17(2): 2100358.

• Drug Design Based on X-ray Crystallography
• Thermodynamic Characterization of Biomolecular Interactions
• Binding Affinity Measurement by MST
• Protein Stability Evaluation by TSA
• Fragment Screening Based on WAC
• Hit Identification by NMR-based Techniques
• Qualification of Targeting Protein-Protein Interactions
• Measurement of Ligand Binding Kinetics
• Identification of Small-Molecule Aggregation
• Analysis of Free Energy Calculations
• Modeling of Scaffold Hopping Transformations
• Cellular Imaging Service for Drug Discovery
• AFM-Based Biophysical Analysis of Drugs