As a company with technological innovation at its core, CD BioSciences is committed to developing methods for visualizing electrical and biochemical activity in neurons to help customers observe the dynamic events occurring within neurons. Our scientific services, including the visualization and manipulation of neural activity, will accelerate our clients' research progress in the field of neuroscience.

Introduction

While methods for visualizing the structure and localization of cells and proteins are well presented for studying the activity that takes place in neurons, however, these methods do not fully reveal the dynamic electrical and biochemical activity found within cells. To observe the dynamic events occurring within neurons, the development of techniques to visualize neural function has important implications.

The first optical recordings of neuronal activity were obtained by scientists from invertebrate motor nerves by the use of a tungsten lamp, a stimulating electrode, and a photocell. In recent years, with the rapid development of technology in the field of biophysics, a variety of methods for visualizing electrical and biochemical activity in neurons have been developed. The combination of laser scanning microscopy with fluorescent dyes and genetically engineered optical sensors has reportedly been successful in obtaining recordings from hundreds of neurons simultaneously at high resolution.

Fig.1 Example of a voltage-sensitive dye imaging experiment. (Carter, 2015)

Our Services

We have established techniques for visualizing neural activity that allow simultaneous viewing of neural activity with single-neuronal resolution or thousands of neurons, while examining the spatial relationships of these neurons. In addition, we offer manipulation of neural activity to help clients study the role that activity plays in shaping neuronal circuits.

• Static measures of activity and function

• Measuring neural activity using immediate early genes.
• Measuring cell proliferation with thymidine analogs.
• Measuring protein trafficking with a pulse-chase labeling.

• Visualizing dynamic neural activity

• Voltage sensors (voltage-sensitive dyes, genetically encoded voltage indicators).
• Calcium sensors (calcium-sensitive dyes, genetically encoded calcium indicators).
• Synaptic transmission sensors (FM dyes, synaptopHluorin).

• Visualizing protein function
• Manipulation of Neuronal Activity

• Optical manipulation of neuronal activity.
• Manipulation of neuronal activity using optogenetics.
• Manipulation of activity in developing neural circuits.

Advantages of Us

• Professional researchers with rich experience
• Optimized scientific scheme design
• Powerful bioinformatics analysis software
• Reliable after-sales service

With a wealth of service experience gained in neuroscience, CD BioSciences has developed a variety of techniques for visualizing and manipulating neural activity to help clients study how proteins determine the properties of neurons, and how neurons determine the properties of circuits and behavior. If you are interested in our services, please feel free to contact us.

Reference

1. Carter, M.; Shieh, J. C. Guide to research techniques in neuroscience. Academic Press. 2015

• Whole-Brain Imaging Based on Biophysical Technology
• Detection of Neurotransmitters
• Analysis of Neurotransmitter Release
• Electrophysiological Measurements of Neurons
• Analysis of Synaptic Transmission and Plasticity
• Analysis of 3D Neural Morphology
• Structural and Functional Analysis of Neural Circuits
• Biophysical Modeling of Neural Systems