Pollen tube growth is a complex kinetic process that extends rapidly in an oscillatory manner through extreme forms of polarised growth, and tip growth. It plays an important role in the sexual reproduction of higher plants. At the same time, it also provides an exciting system for the study of the spatiotemporal control of polarized cell growth. The growth process of pollen tubes includes many aspects, the most important of which are the dynamics of the pollen tube cytoskeleton and cytoplasmic motility.

Because of the specificity of pollen tube growth behavior, it is considered an ideal experimental model system for in-depth studies of plant cell wall construction, signal transduction and intercellular communication. As an important cellular system in plant fertilization, the study of the cell biology and molecular biology of pollen tubes is of great importance both in theory and in production practice. In recent years, new approaches in the field of biophysics, including several emerging labeling and imaging techniques, have provided powerful tools to study the complex physiological activities of pollen tubes in vivo during growth. With the development and widespread application of these new techniques, more detailed structures within plant pollen tubes and their growth mechanisms will be revealed.

Fig.1 Structural regulation of pollen tube growth. (Guan, 2013)

Our Services

Our services include but are not limited to:

• Analysis of Pollen Tube Growth Behavior
  We have developed multiple design strategies to expose in vitro-grown pollen tubes to a range of experimental assays to study their behavior and their mechanical properties.
• Determination of Pollen Tube Extension
  We are committed to developing simple and rapid strategies that can be used to help customers quantify pollen tube extension, offering the possibility of performing comparative chemical screens.
• Analysis of Pollen Hydration
  In conjunction with pollen viability assays and time-series imaging analysis of pollination, we offer pollen hydration analysis services to help clients explore the key players in water transport from papilla cell to pollen during pollination.
• Imaging of Actin Cytoskeleton in Pollen Tubes
  We provide clients with high-resolution imaging of the actin cytoskeleton in pollen tubes to study the dynamics of actin filaments in pollen tubes.
• Live Cell Imaging of Pollen Tubes
  We are dedicated to providing high-quality live-cell imaging of pollen tubes to help our customers explore the characteristic growth kinetics of pollen tube formation.
• Measurement of Cytomechanical Forces on Growing Pollen Tubes
  We provide our customers with an accurate assessment of the mechanical properties of the cell wall along the length of the pollen tube to reveal the secrets of pollen tube growth mechanics.
• Modeling of Pollen Tube Growth
  We provide modeling services for pollen tube growth in order to obtain important information on the expansion pressure, cellular mechanical properties and non-linear dynamics of pollen tube growth.
• AFM-Based Biophysical Analysis of Pollen Tubes
  We provide biophysical analysis services of pollen tubes based on AFM technology to obtain important information such as morphological and structural characteristics of pollen tube surfaces, chemical composition and molecular structure of pollen tube surfaces, interactions between pollen tubes and other biomolecules (e.g., interactions between pollen tubes and cell walls, pollen grains and compounds), and pollen tube growth rates and directions.

Our Advantages

• Different technical methods
• Delivery of detailed reports at the end of the project
• High-quality services and competitive pricing
• Customer service orientation

Molecular biologists, biophysicists and bioinformaticians at CD BioSciences are well-prepared and positioned to collaborate and provide a wide range of scientific services in the field of pollen tip growth. Every project will be treated seriously and guaranteed a high degree of sensitivity and accuracy. If you are interested in our services, please feel free to contact us.

Reference

1. Guan, Y.; et al. Signaling in pollen tube growth: crosstalk, feedback, and missing links. Molecular plant. 2013, 6(4): 1053-1064.

• Biophysical Analysis for Photosynthesis
• Biophysical Analysis for Plant Cell Walls
• Biophysical Analysis for Plant Biomechanics
• Biophysical Analysis for Root Growth